高速液体クロマトグラフィーによる塩酸ヨヒンビン及びメチルテストステロンの定量(<特集>医薬品の分析)

概要

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A method for the simultaneous determination of yohimbine hydrochloride and methyltestosterone in a tablet by high performance liquid chromatography (HPLC) was established. Yohimbine hydrochloride and methyltestosterone in the tablet were extracted with methanol. Chromatographic separation was achieved on a reversed phase column packed with Hitachi Gel #3056 using a mobile phase of methanol-water-0.2 M ammonium phosphate(pH 3) (70 : 30 : 2 in v/v). The eluate was monitored with an ultraviolet detector at a wavelength of 270 nm. Determination was carried out successfully by using homosulfamine as an internal standard. Recoveries of yohimbine hydrochloride and methyltestosterone from mixture of standards were quantitative. The relative standard deviation for them were less than 2.0%. By this chromatographic method, these components in commercial tablets could be simultaneously determined without any interference. The method is rapid with a total analysis time of within 10 min.
社団法人日本分析化学会の論文
1986-03-05