カラムスイッチング高速液体クロマトグラフィーによる血清中のテオフィリン及び抗てんかん薬の迅速定量

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The rapid determination of theophylline and antiepileptic drugs (phenobarbital, carbamazepine, phenytoin and valproic acid)in human serum using a combination of a short pre-treatment column (TSKprecolumn BSA-ODS/S) and a short analytical column (TSKgel Super-ODS) was investigated. Under the optimized analytical conditions, theophylline and four antiepileptic drugs were eluted within 14 min. The relationship between the sample amount and the peak areas of five drugs indicated good linearity over the range from 1.0 to 500 mg/l (valproic acid) and 0.2 to 110 mg/l (other drugs), respectively. Five drugs from spiked serum were recovered quantitatively. The repeatability and reproducibility of the peak areas of standard and spiked serum were less than 1.6%. The drug concentrations in human serum determined by a enzyme immunoassay method and a column-switching method showed good agreement. Therefore, this column-switching method is very suitable for therapeutic drug monitoring (TDM) in biological fluids.
社団法人日本分析化学会の論文
2000-03-05