土壤中における放射菌の分布 : (第6報) MicrobisporaおよびStereptoporangium属の選択的平板分離法(その1)

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A plate culture method effective for both selective isolation and enumeration of Microbispora (Mb) and Streptosporangium (Ss) strains in soil was studied. The procedure characteristic of this method is a dry heating of soil samples at a high temperature prior to the preparatio of soil-water suspensions.Soil samples were dirst dried at room temperature and ground in a mortar, then heated in a hot air oven (120℃) for one hour. This treatment always reduced the number of bacterial colonies drastically and also streptomycetes considerably. But Mb and Ss, strains were reduced only slightly by this treatment. Therefore, by this the isolation frequencies of actionomycetes of these genera were remarkably increased.A synthetic medium, AV-Agar (Table 2), was used for this plate culture. Generally, B-vitamins were essential for the growth of these strains, and lower concentrations of nutrients were favorable for the growth of aerial mycelia. An antifungal substance indespensable for this culture, while an antibacterial substance was useful only when the plates were incubated at 50℃.The plants were examined under microscope after 40 day incubation at 30℃ or 20 day incubation at 50℃.The numbers of actionmycetes of these genera in the soils examined were over ten thousands per gram of dry soil. Moreover, an addition of turf to soils markedly increased the number ofthe total actionmycetes and Microbispora. It was also found that thermophilic strains of Microbispora were commonly distributed all over in the soils.
社団法人日本生物工学会の論文
1969-08-25

土壤中における放射菌の分布 : (第6報) MicrobisporaおよびStereptoporangium属の選択的平板分離法(その1)

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