Saccharomycodes ludwigiiの単胞子培養株について : (第3報) 胞子間および栄養細胞間交雑による雑種の形成とその四分子解析

概要

論文の詳細を見る
Further studies have been carried out on the nonagglutinative (Z type) hybrids obtained by the crossing of spores and of vegetative cells in single-spore cultures (clones) of Saccharomycodes ludwigii with agglutination types opposing each other(X and Y type). The tetrads of 8 hybrids and their progenies (i.e. segregants) were analysed.Vegetative cells in hybrids (F_1) derived from spore-to-spore copulation were considered as either a heterozygous diploid (F_1-203), or a tetraploid (F_1-103), while other vegerative cells derived from cell conjugations were all tetraploids (F_1-113 and -213). The Z type cells segregated into the X and Y types in the following generation, i.e. F_2 or F_3. However, one of them segregated into the X, Y and Z types even in F_4. This suggested a diploid had developed into tetraploid (F_1-, F_2- and F_3-103) cultures by way of its intra-cellular self-diploidization. It was also found that in the F_1-113 and F_2-213 there existed tetrads which segregated irregularly (i.e. 2X2Z).All of the Z type clones sporulated aboundantly; these spores were always viable in the diploid(F_1-203) cultures, while only a fer spores were reproducible in tetraploid(F_1-103,-113 and -213) cultures. On the other hand, all clones of the X and Y types were scarcely sporiferous or did not sporulate at all. However, their ascospore tetrads examined were always viable.From these results, it was confirmed that the mutual agglutinaiton found in the clones of Sd. ludwigii O-17 (I) and O-81 (II) was a sexual character promoting the plasmogamy and relating closely to the mating type.
社団法人日本生物工学会の論文
1969-11-25

Saccharomycodes ludwigiiの単胞子培養株について : (第3報) 胞子間および栄養細胞間交雑による雑種の形成とその四分子解析

スポンサーリンク

概要

著者

関連論文

スポンサーリンク