光照射を受けたYeast Nitrogen Base (1% Glucose)の各種酵母に対する毒性

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Bacto-Yeast Nitrogen Base plus 1% glucose medium exposed to light was toxic for yeast growth. Sensitivity to the toxic photoproducts was different among yeast strains.In the medium exposed to sunlight for 4 h, no growth was observed with Saccharomycodes ludwigii, Torulaspora delbrueckii S-90 (Saccharomyces rosei), Kloekera apiculata, and Candida cantarellii, and one-week-late growth with Pichia membranaefaciens d-1. Sensitivity of the other strains was in the following order. Sacch. cerevisiae S-74 (bayanus)=a few days-late growth>Sacch. cerevisiae Kyokai no. 7>T'spora delbrueckii S-65 (Sacch. fermentati)>Sacch. cerevisiae OC-2,Hansenula anomala>P. guilliermondii, P. membranaefaciens K-18,Kl. africana, Schizosaccharomyces pombe, C. stellata, and Rhodotorula rubra.The most sensitive strain, S'codes ludwigii, did not grow in medium exposed to fluorescent light for 2 h (18 W×2,10 cm).Tryptophan (try) was necessary and riboflavin (rib) accelerated the formation of the toxic photoproducts in the medium. One of the products, H_2O_2,was at the 60-70 μM level (sunlight, 4 h) in a aqueous solution of try and rib at the same concentrations as in the medium, but MIC of H_2O_2 alone on these yeasts in the medium was at the 2-3 mM level.
公益社団法人日本生物工学会の論文
1989-11-25