Saccharomycodes ludwigiiの単胞子培養株について : (第1報)胞子形成能ならびに相互凝集性

概要

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Ascospore tetrads were isolated from the yeast, Saccharomycodes ludwigii Hansen by micromanipulation. It was found that each of the 4 single-spores isolated from an ascus was viable, except some asci in one parental strain (I), of which 2 out of the 4 single-spore isolates lost their viability. Almost all clones obtained from the 4 parental strains (I-IV) sporulated singly during the period of F_1 to F_3,but the amount of spores produced was generally small except heterozygous clones.An intensive mutual agglutination was observed in certain combinations of the crossing of the clones (F_1) derived from the parental strain I and/or II. Some clones of the strain I did not agglutinate with any other clones at the stage of F_1,but they segregated to have their opposite types of agglutination by further single-spore isolation, i.e. F_2. The crossing between vegetative cells with agglutination types opposing each other resulted in production of a few heterozygous hybrids, (not agglutinative) but highly sporiferous. The results suggested that these mutually agglutinative reactions in S. ludwigii might be a heterothallic character of the clones. These reaction may possibly be comparable with the mating types, concealed mating types of unbalanced homothallic strains and sexual agglutination in Saccharomyces.In the meanwhile, a self-agglutination was observed in the clones of the other parents (III and IV).
社団法人日本生物工学会の論文
1968-05-25