Saccharomycodes ludwigiiの二倍体交雑株における体細胞組み換えおよび半数体化の徴候 : Saccharomycodes ludwigiiの単胞子培養株について(第8報)

概要

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Sectored colonies were isolated from the diploid clones (h^<+>/h^<-> ADE/ade or h^<+>/h^<-> ade/ADE) in a non-sporulating condition at a frequency of 10^<-4> by spreading on a plate of Wickerham's minimal agar supplemented with adenine. Adenine-dependent pink and independetn white clones obtained from the sectors were capable of sporulation and were subiected to tetrad analysis . The genotypes of these segregants were shown to be consitent with homozygous ade and ADE (h^<+>/h^<-> ade/ade and h^<+>/h^<-> ADE/ADE). A few haploid segregants were also isolated as agglutinative pink (h^<-> ade) or white (h^<+> ADE and h^<-> ADE) colonies on the same plate at a frequency of 10^<-5>. It therefore seems likely that these homozygous diploid and haploid segregants arise from the occurrence of spontaneous mitotic recombination and vegetative haploidization, respectively.
社団法人日本生物工学会の論文
1973-05-25