Saccharomycodes ludwigiiの単胞子培養株について : (第7報) 紫外線により誘導されたアデニンまたはウラシル要求変異株

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In order to obtain genetic markets other than the heterothallic agglutination types (h^+ and h^- which were expressed by the symbols X/Y), isolation of auxotrophic mutants was carried out by treating the aggllutinative standard wild-type strains with ultraviolet irradiation.Two adenine dependent mutants (pink) of both agglutination types (h^+ and h^-) and one uracil dependent mutant of agglutination type h^- were isolated by a replica plating technique from 1.2×10^4 colonies tasted. with an approximately 0.1% survival rate.These mutants were capable of sporulation; two of them produced 4 spored asci and another one produced mostly 2 spored asci. It was observed that each consisted of mixed cell populations, similar to the wild-type strains described in our previous papers.The agglutination types and the auxotrophic markers in the hybrids among these mutants and the prototrophic strains of opposite types (h^+ or h^-) were determined, and the representative prototrophic diploid hybrids were subjected to tetrad analysis.Each auxotrophic mutant trait showed the 2 : 2 monogenic ratio (i.e., 2 ade^+ : 2 ade^- or 2 ura^+ : 2 ura^-) in all tested asci. Tetrad distribution of each gene pair between the agglutination types and one of these mutant markers showed the parental ditype or non-parental ditype in all asci so far tested; no tetra type ascus was obteined.The results suggested that these auxotrophs are monogenic mutants and each mutant phenotype is controlled by the recessive allele.
社団法人日本生物工学会の論文
1972-08-25

Saccharomycodes ludwigiiの単胞子培養株について : (第7報) 紫外線により誘導されたアデニンまたはウラシル要求変異株

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