ビタミンB_1強化食品に関する研究 (第3報) : 強化食品中のO, S-ジベンゾイルビタミンB_1の浸出について

概要

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A study on the extraction of O, S-dibenzoylthiamin (DBT) in the enriched foods was carried out and the following results were obtained.1) Above 90% of DBT in the enriched raw foods were recovered by extracting with five times 0.1N hydrochloric or sulfuric acid at 50℃.2) The digestion by Takadiastast B (Takadiastase for thiamine assay, Sankyo Co., Ltd., Tokyo) at pH 5.6〜6.0 was most effective to the extraction of DBT.3) The treatment by pepsin before the digestion by Takadiastase B enhanced the protein digestion, but resulted in the inhibition of DBT-ase activity.4) After the digestion by Takadiastase B, the extraction of DBT remained in the residue was effected by 0.1N acetic or citric acid which was found to be more suitable for the extraction that 0.1N hydrochloric or sulfuric acid.
社団法人日本生物工学会の論文
1960-06-15