ビタミンB_1強化食品に関する研究 : (第10報) アルカリ分解によるDibenzoylthiamine定量法の検討

概要

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The method of determining dibenzoylthiamine (DBT) in foods enriched with it where it was extracted with 70% ethylalcoholic-0.1 N hydrochchloric acid (EtOH sol.) after which the EtOH sol of DBT was hydrolysed with alkali, was studied.DBT added to food was more easily extracted with EtOH sol. than with 0.1N hydrochloric or sulfulic acid. But much more fluoresent substance which disturbed the fluorometrical measurement was extrated with EtOH sol. than Takadiastase B digestion.Fluorescence degree of the products which were obtained by alkali-hydrolysis of DBT in EtOH sol. was constant in each examination and was almost equal to that of equivalent mole of thiamine-hydrochloride.It was impossible to hydrolyse thiamine-pyrophosphate by this method, and when EtOH sol was used for solvent the recovery of thiamine-hydrochloride was not perfect when using the usual treatment for purification.Based on these experiments, it was considered that this determination method of DBT was suitable for the foods which were enriched with a large quantity of DBT and also contained less fluorescent substances.
公益社団法人日本生物工学会の論文
1964-02-15