ビタミンB_1強化食品に関する研究 : (第1報) ビタミンB_1有機塩の定量

概要

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It has been reported that the organic salts of thiamine which are almost insoluble in water have to be dissolved by boiling in 0.1N-HCl containing 25% KCl (w/v) before the determination. But solution of a thiamine containing 25% KCl can be hardly purified by the usual method. Therefore, it was attempted to devise a method, which is able to purify the sample solution by the usual method.By the paper partition chromatography (chloroform : methanol=5 : 1), it has been found that the almost insoluble thiamine dicetylsulfate and dilaurylsulfate were converted into thiamine hydrochloride by boiling in a 0.1N-HCl solution for 20〜30 minutes. So the organic salts of thiamine that are almost insoluble in water can be also determined by the usual method.
社団法人日本生物工学会の論文
1960-05-15