大腸菌のγ-グルタミルトランスペプチダーゼ

概要

論文の詳細を見る
γ-Glutamyltranspeptidase (GGT, EC 2.3.2.2) of Escherichia coli K-23 consists of one 39kDa large subunit and one 20kDa small subunit. The amino acid sequence of E. coli GGT has rather high similarity to eukaryotic GGTs. E. coli utilizes a γ-glutamyl amino acid as an amino acid source, for which GGT is essential. Without GGT activity E. coli cannot utilize extra-cellular glutathione as cysteine source nor glycine source. GGT was recovered from the culture broth of periplasmic leaky mutant harboring the multicopy plasmid with ggt gene and isolated simply by ammonium sulfate fractionation and Chromatofocusing column chromatography. Several γ-glutamyl amino acids were synthesized by E. coli GGT. Crystallographic analysis and site-directed mutagenesis of E. coli GGT were also performed.
日本ビタミン学会の論文
1994-09-25