Thiamine Disulfideに関する研究 : (VL)シロネズミ肝臓におけるThiamine-S-sulfonateならびにCysteine-S-sulfonateの還元機作

概要

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As for the nonenzymatic reaction of thiamine-S-sulfonate (B_1SSO_3H) and cysteine-S-sulfonate (CySSO_3H) with GSH, the apparent second order rate constants were estimated under physiological conditions. It was revealed that the mixed disulfide forming reaction (1) was the rate limitting step in the case of B_1SSO_3H. B_1SSO_3H(CySSO_3H) + GSH→B_1SSG^*(CySSG)^<**> +H_2SO_3 (1) B_1SSG(CySSG) + GSH→B_1SH(CySH) +GSSG (2) ^*thiamine-glutathione disulfide ^<**>cysteine-glutathione disulfide An enzyme catalizing these reactions (1,2) was demonstrated in the supernatant fraction of rat liver extracts. This GSH-dependent enzyme was supposed to be different from the previously reported GSH-B_1 derivatives transhydrogenase. Mechanisms of the conversion of B_1SSO_3H to thiamine and of CySSO_3H to cysteine were discussed.
日本ビタミン学会の論文
1974-08-25