ビタミンB_<12>の酸化触媒作用に関する研究 : (IX)ビタミンB_<12>による低分子SH化合物共存下の酵母アルコール脱水素酵素の阻害

概要

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Cyanocobinamide (Factor B) and aquocobalamin (OH-B_<12>) inhibited yeast alcohol dehydrogenase (YADH). This inhibition was remarkably increased by the addition of GSH. GSSG, however, did not affect the inhibition of YADH with OH-B_<12> or Factor B. The inhibition with Factor B and GSH was suppressed by the addition of catalase. Completely inhibited YADH, obtained by the gel filtration of a reaction mixture, was found to lose about 7 SH groups (mol/YADH mol) and to release the GSH bound to the enzyme by CySH reduction. The inhibited YADH was partially reactivated by CySH. From these results it was concluded that OH-B_<12> and Factor B catalyze the formation of mixed disulfides between small molecular thiols and YADH.
日本ビタミン学会の論文
1975-06-25