固定化タンニンによる清酒の連続清澄化

概要

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As finning of sake is generally carride out by a batch method employing a precipitation method with persimmon juice or an enzymatic method with proteases, it requires much time, labor, and spaces for operation. To overcome these disadvantages, we previously reported a method of continous finning of fresh sake using immobilized tannin prepared by coupling BrCN-activated chinese gallotannin to aminohexyl cellulose, and clarified the possibility of use of this adsorbent for a practical purpose.In order to obtain a more useful adsorbent, we prepared the immobilized tannin by a new method, that is, a method coupling chinese gallotannin to epichlorohydrin-activated aminohexyl cellulose. This adsorbent was found to be more effective for finning of sake than the previous adsorbent. The amount of sake which could be finned by 1 g of adsorbent was bout 4 l. The capacity of finning of sake by the adsorbent increased at a low temperature, but was not influenced by the flow rate of fresh sake or the column length. When the columu packed with the adsorbent was regenerated by 0.01 N hydrochloric acid, repeated use of more than 40 times was possible without loss of finning capacity for sake. The specific resistance of filtration of this adsorbent increased as the pressure drop increased. This tendency was high at more than 0.5 atm.
社団法人日本生物工学会の論文
1979-05-25