ホップ矮化病に関する研究第2報 : 病原ウイロイドについて

概要

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As the indicator plant of hop stunt disease (HSD) agent, cucumber plants were used to investigate the properties of HSD agent. HSD agent incrude sap from diseased cucumber plants was not in activated after heating at 84 C for 10 min, lost its infectivity when diluted 1/10,000, and kept for one day at 20 C, but not after three days at 4 C. HSD agent was easily extractable with buffers of high ionic strength (0.5 M K_2 HPO_4). The agent remained in the supernatant of high speed centrifugation (113,000×g, 2 hours), was extracted with phenol and precipitated with ethanol. The agent was inactivated with RNase, but not with DNase. Fraction showing high infectivity was detected between 6 S and 9 S by the 5-20% sucrose density gradient centrifugation. The results indicate that HSD agent is a low molecular weight ribonucleic acid, a viroid.
日本植物病理学会の論文
1978-12-25