Synthesis of Methyl Ursodeoxycholate via Microbial Reduction of Methyl 7-Ketolithocholate with Eubacterium aerofaciens JCM 7790 Grown on Two Kinds of Carbon and Hydride Sources, Glucose and Mannitol

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概要

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• Eubacterium aerofaciens JCM 7790, which produces 7β-hydroxysteroid dehydrogenase [7β-HSDH], efficiently catalyzed the reduction of methyl 7-ketolithocholate [Me-7KLCA] to afford methyl ursodeoxycholate [Me-UDCA] in an anaerobic interface bioreactor. For the high accumulation of Me-UDCA, the best supplement of carbon and hydride source to the ABCM medium (glucose content, 0.3 wt.%) was screened because glucose strongly repressed 7β-HSDH production. The supplementation of cellobiose, mannitol, sodium gluconate, and sodium pyruvate was effective to improve the production of Me-UDCA, although many mono-and disaccharides, such as fructose, sucrose, and lactose, strongly repressed 7β-HSDH activity. A phosphate buffer was used as the aqueous phase in the carrier to avoid the lowering of medium pH in the carrier caused by the accumulation of organic acids via fermentation by E.aerofaciens. Thus, the productivity of Me-UDCA was elevated by the combination of glucose and mannitol as carbon and hydride sources and by using a phosphate buffer.

• 社団法人日本生物工学会の論文
• 2001-02-25

著者

• 須貝 威

  慶應義塾大学理工学部

• Oda S

  Analytical Center Kansai Paint Co. Ltd.

• Oda S

  Kansai Paint Co. Ltd. Kanagawa Jpn

• Oda Shinobu

  Technical Research Laboratory Kansai Paint Co. Ltd.

• Ohta H

  Okayama Univ. Okayama

• Ohta H

  Department Of Bioscience And Informatics Center For Life Science And Technology Keio University

• Ohta H

  Department Of Chemistry Faculty Of Science And Technology Keio University

• Ohta H

  Department Of Chemistry Keio University

• Ohta Hiromichi

  Department Of Chemistry Faculty Of Science And Technology Keio University

• Ohta H

  Keio Univ. Kanagawa

• Sugai Tomoharu

  Department Of Bioresource Engineering Faculty Of Agriculture Yamagata University

• SUGAI Takeshi

  Department of Chemistry, Faculty of Science and Technology, Keio University

• Ohta H

  Department Of Food And Nutrition Nakamura Gakuen University

• Ohta Hiromichi

  Department Of Applied Chemistry School Of Engineering Nagoya University

• Okazaki Hanako

  Department Of Chemistry Keio University

• Sugai Takeshi

  Department Of Agricultural Chemistry The University Of Tokyo

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