Purification and Characterization of Arylmalonate Decarboxylase from Achromobacter sp. KU1311(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
- 論文の詳細を見る
We have isolated, purified and characterized arylmalonate decarboxylase (AMDase ; EC 4.1.1.76). This is an unique enzyme that gives optically pure arylpropionates from the corresponding arylmalonates. Recently, we have screened similar enzyme producers from soil samples and succeeded in isolating Achromobacter sp. KU1311. The gene encoding the enzyme was cloned and sequenced. The AMDase gene consists of 720 nucleotides, which specifies a 240 amino acid protein with a relative molecular mass of 24, 735. This enzyme was purified and its characteristics were compared with those of the hitherto known enzyme from Alcaligenes bronchisepticus KU1201.
- 社団法人日本生物工学会の論文
- 2007-10-25
著者
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MIYAMOTO Kenji
Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University
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TAMURA Keisuke
Department of Particle and Astrophysics, Nagoya University
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Ohta Hiromichi
Department Of Chemistry Faculty Of Science And Technology Keio University
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Ohta Hiromichi
Department Of Biosciences And Informatics Keio University
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Tamura Keisuke
Department Of Biosciences And Informatics Faculty Of Science And Technology Keio University
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Tamura Keisuke
Department Of Astrophysics Nagoya University
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Ohta Hiromichi
Department Of Applied Chemistry School Of Engineering Nagoya University
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Yatake Yoshito
Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University
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Terao Yosuke
Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University
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Terao Yosuke
Department Of Biosciences And Informatics Keio University
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Terao Yosuke
Department Of Biosciences And Informatics Center For Life Science And Technology Keio University
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Yatake Yoshito
Department Of Biosciences And Informatics Faculty Of Science And Technology Keio University
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Miyamoto Kenji
Department Of Biosciences And Informatics Keio University
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Ohta Hiromichi
Department Of Biosciences And Informatics Center For Biosciences And Informatics Keio University
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Miyamoto Kenji
Department Of Biosciences And Informatics Center For Biosciences And Informatics Keio University
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