MEASUREMENT OF HUMAN PROLACTIN MESSENGER RNA IN DECIDUAL TISSUES USING COMPLEMENTARY DNA PROBE CLONED IN M13mp9 BACTERIOPHAGE
スポンサーリンク
概要
- 論文の詳細を見る
A human prolactin (PRL) CDNA clone was digested with restriction enzyme Pst I and the resultant fragments were cloned into bacteriophage M13mp9.Single stranded recombinant DNAS having a coding strand of the PRL cDNA were selected by hybridization with ^<125>I-labeled PRL mRNA obtained from human prolactinoma tissue. One of the single stranded recombinant DNAS was purified by agarose gel electrophoresis and labeled with ^<125>I to a specific activity of 1.4 x 10^8cpm per μg of DNA. The probe could be successfully used in RNA dot hybridization. Analysis of poly (A) RNAS from prolactinoma, liver and placental tissues revealed that this probe was specific to PRL mRNA sequence.Hybridization of poly (A) RNA from decidual tissue to this probe revealed the presence of PRL mRNA sequence. However, PRL mRNA in decidua was at least 20,000 times less than that in pituitary prolactinoma.
- 社団法人日本産科婦人科学会の論文
- 1986-02-01
著者
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Seo Hisao
名古屋大学環境医学研究所
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Seo H
Nagoya Univ. Nagoya Jpn
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Seo Hisao
^*department Of Endocrinology And Metabolism Research Institute Of Environmental Medicine Nagoya Uni
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Miyamoto Norihiro
Department Of Endocrinology And Metabolism The Research Institute Of Environmental Medicine Nagoya U
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Seo Hisao
Research Institute Of Environmental Medicine Nagoya University
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Seo Hisao
The Research Institute Of Environmental Medicine Nagoya University
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Seo Hisao
Department Of Endocrinology And Metabolism The Research Institute Of Environmental Medicine Nagoya U
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Tomoda Yutaka
Department Of Obstetrics And Gynecology Nagoya University School Of Medicine
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Tomoda Yutaka
Department Of Biopharmaceutics Kyoto Pharmaceutical University
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SUGANUMA NOBUHIKO
Department of Obstetrics and Gynecology, Nagoya University School of Medicine
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NARITA Osamu
Department of Obstetrics and Gynecology, Nagoya University, School of Medecine, Nagoya
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Matsui N
Department Of Obstetrics And Gynecology And Research Institute Of Environmental Medicine Nagoya Univ
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MATSUl Nobuo
The Research Institute of Environmental Medicine, Nagoya University
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Matsui Nobuo
Department Of Endocrinology And Metabolism The Research Institute Of Environmental Medicine Nagoya U
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Matsul Nobuo
The Research Institute Of Environmental Medicine Nagoya University
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Narita Osamu
Department Of Obstetrics And Gynecology Nagoya University School Of Medicine
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Tomoda Yutaka
Department Of Obstetrics And Gynecology And Research Institute Of Environmental Medicine Nagoya Univ
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Suganuma N
Toyohashi Municipal Hospital Toyohashi Jpn
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Suganuma Nobuhiko
Department Of Obstetrics And Gynecology Nagoya University School Of Medicine
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Suganuma Nobuhiko
Infertility Center Toyohashi Municipal Hospital
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Matsui Nobuo
Department Of Endocrinology And Metabolism The Research Institute Of Environmental Medicine Nagoya U
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Narita Osamu
Department Of Obstetrics And Gynaecology Nagoya University School Of Medicine
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Suganuma Nobuhiko
Department of Human Health Science, Graduate School of Medicine, Kyoto University
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