Spatial and Intracellular Distribution of the Endogenous Calcineurin-Inhibitory Proteins, ZAKI-4, in Mouse Brain

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概要

• 論文の詳細を見る

• ZAKI-4 is a thyroid hormone-responsive gene which encodes two isoforms, ZAKI-4 α and β, both of which belong to a family of proteins that inhibit calcineurin activity. Calcineurin is a calcium/calmodulin-dependent phosphatase and is known to play crucial roles in brain development and function. Using in situ hybridization and immunohistochemistry, the present study aimed to demonstrate regional distribution of ZAKI-4 mRNAs and proteins in the mouse brain. Both ZAKI-4 mRNA isoforms showed similar spatial expression in the brain. Although they were widely expressed throughout the entire brain, the highest expression was observed in neurons in olfactory bulb, hippocampus and cerebellum. Consistent with the expression of ZAKI-4 mRNA isoforms, ZAKI-4 proteins were widely, but not evenly, distributed in the brain ; the most intense immunoreactivity was found in the olfactory bulb, cerebral neocortex, hippocampus and cerebellum. As for the subcellular localization, ZAKI-4 immunoreactivity was confined to neuronal somata, with higher expression in the soma than in dendrites, and was not detected in glia. The double immunostaining of ZAKI-4 proteins and calcineurin revealed that they were co-localized in the periphery of the soma and dendrites of neurons. These results indicate that ZAKI-4 proteins are localized in the area where they are able to inhibit calcineurin activity.

• 日本組織細胞化学会の論文

著者

• Murato Y

  Division Of Cardiology Department Of Medicine And Clinical Science Yamaguchi University Graduate Sch

• Murata Yoshiharu

  Division Of Cardiology Department Of Medicine And Clinical Science Yamaguchi University Graduate Sch

• Kambe Fukushi

  名古屋大学環境医学研究所

• Yamazaki Masatoshi

  Division Of Cardiology Department Of Medicine And Clinical Science Yamaguchi University Graduate Sch

• 高岸 芳子

  名古屋大学環境医学研究所

• Takagishi Yoshiko

  Department Of Teratology And Genetics Research Institute Of Environmental Medicine Nagoya University

• Takeuchi Yoko

  Department Of Genetics Research Institute Of Environmental Medicine

• Takagishi Yoshiko

  Research Institute Of Environmental Medicine Nagoya University

• 早坂 静

  名古屋大・環境医学研究所

• Hoshino Shin

  Division of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya

• Takagishi Yoshiko

  Division of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya

• Kanou Yasuhiko

  Division of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya

• Hayasaka Shizu

  Division of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya

• Hattori Kimihiko

  Division of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya

• Kambe Fukushi

  Division of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya

• Seo Hisao

  Division of Molecular and Cellular Adaptation, Research Institute of Environmental Medicine, Nagoya

• Murata Yoshiharu

  Department Of Civil Engineering College Of Engineering Nihon University

• Murata Y

  Department Of Teratology And Genetics Research Institute Of Environmental Medicine Nagoya University

• Seo Hisao

  名古屋大学環境医学研究所

• Seo H

  Nagoya Univ. Nagoya Jpn

• Seo Hisao

  ^*department Of Endocrinology And Metabolism Research Institute Of Environmental Medicine Nagoya Uni

• Seo Hisao

  Research Institute Of Environmental Medicine Nagoya University

• Seo Hisao

  Department Of Endocrinology And Metabolism The Research Institute Of Environmental Medicine Nagoya U

• Hoshino Shin

  Division Of Molecular And Cellular Adaptation Research Institute Of Environmental Medicine Nagoya Un

• Kambe F

  Department Of Endocrinology And Metabolism Research Institute Of Environmental Medicine Nagoya Unive

• Kanou Y

  Division Of Molecular And Cellular Adaptation Research Institute Of Environmental Medicine Nagoya Un

• Kanou Yasuhiko

  Department Of Genetics Research Institute Of Environmental Medicine Nagoya University

• Kanou Yasuhiko

  Division Of Molecular And Cellular Adaptation Research Institute Of Environmental Medicine Nagoya Un

• Hayasaka Shizu

  Department Of Genetics Research Institute Of Environmental Medicine Nagoya University

• Hayasaka Shizu

  Research Institute Of Environmental Medicine Nagoya University

• Seo H

  Department Of Endocrinology And Metabolism Division Of Molecular And Cellular Adaptation Institution

• Kambe Fukushi

  Department Of Endocrinology And Metabolism Research Institute Of Environmental Medicine Nagoya Unive

• Hattori Kimihiko

  Division Of Molecular And Cellular Adaptation Research Institute Of Environmental Medicine Nagoya Un

• Hayasaka Shizu

  名古屋大学環境医学研究所

• Hayasaka Shizu

  Res. Inst. Environ. Med. Nagoya Univ.

• Murata Yoshiharu

  Department Of Endocrinology And Metabolism Research Institute Of Environmental Medicine

• Yamazaki Masatoshi

  Division Of Cardiology Shizuoka Saiseikai General Hospital

• Yamazaki Masatoshi

  Research Institute Of Environmental Medicine Nagoya University

• Murata Yoshiharu

  Division Of Cardiology Department Of Internal Medicine Noto General Hospital

• Sasano Hironobu

  Department Of Pathology Ii Tohoku University School Of Medicine

• Seo Hisao

  Department Of Endocrinology And Metabolism Research Institute Of Environmental Medicine Nagoya Unive

• Yoshida Masaaki

  Division Of Cardiology Department Of Medicine And Clinical Science Yamaguchi University Graduate School Of Medicine

• Takagishi Yoshiko

  Division of Arrhythmia and Electrophysiology, Shizuoka Saiseikai General Hospital

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