Internalization and Dephosphorylation of Connexin43 in Hypertrophied Right Ventricles of Rats With Pulmonary Hypertension

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概要

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• Background Altered expression and distribution of gap junctions might provide substrates for abnormal conduction and arrhythmogenesis in the heart, but little is known about the regulation of gap junctions under pathological conditions. The organization and phosphorylation state of connexin43(Cx43) in ventricular hypertrophy will be investigated. Methods and Results Right ventricular(RV) hypertrophy was induced in rats by treatment with monocrotaline. Subcellular Cx43 distribution was assessed by immunoconfocal and electron microscopy. Immunolabeling of Cx43 was confined to the intercalated disks in the normal ventricular myocytes of control rats, but hypertrophied RV cells from monocrotaline-treated rats showed dispersion of Cx43 immunolabeling over the cell surface and in the cytoplasm; cytoplasmic Cx43 was increased by 〜7-fold(n=15). The Cx43 internalization was confirmed by the double staining of monocrotaline-treated RV tissues for Cx43/wheat germ agglutinin(WGA) and Cx43/ zonula occludens protein-1(ZO-1). Electron microscopy of hypertrophied RVs showed an increase in annular gap junctions immunolabeled with Cx43. Immunoblotting revealed a significant increase in non-phosphorylated Cx43 in hypertrophied RVs(by 〜5-fold, n=8) without changes in the total amount of Cx43. The accumulation of non-phosphorylated Cx43 in hypertrophied RVs was also recognized by immunoconfocal-microscopy with an isoform-specific antibody. Conclusion Ventricular hypertrophy is associated with the dephosphorylation of Cx43 and its translocation from the intercalated disks to intracellular pools, suggesting accelerated gap junction degradation.

• 社団法人日本循環器学会の論文
• 2007-02-20

著者

• Murato Y

  Division Of Cardiology Department Of Medicine And Clinical Science Yamaguchi University Graduate Sch

• Murata Yoshiharu

  Division Of Cardiology Department Of Medicine And Clinical Science Yamaguchi University Graduate Sch

• Uzzaman Mahmud

  名古屋大学環境医学研究所循環器分野

• Kodama Itsuo

  Department of Cardiovascular Research, Research Institute of Environmental Medicine, Nagoya Universi

• HONJO Haruo

  Department of Humoral Regulation Division of Regulation of Organ Function Research Institute of Envi

• KAMIYA Kaichiro

  Department of Humoral Regulation Division of Regulation of Organ Function Research Institute of Envi

• Yamamoto Mitsuru

  名古屋大学環境医学研究所 循環器学

• Yamamoto M

  Department Of Cardiology Mitsubishi-nagoya Hospital

• Kodama Itsuo

  東京医科歯科大学生体材料工学研究所

• Yamazaki Masatoshi

  Division Of Cardiology Department Of Medicine And Clinical Science Yamaguchi University Graduate Sch

• Yamazaki Masatoshi

  名古屋大学環境医学研究所 循環器学

• Honjou Haruo

  名古屋大学 循環器内科学

• Honjou Haruo

  Department Of Humoral Regulation Research Institute Of Environmental Medicine Nagoya University

• Honjo Haruo

  名古屋大学環境医学研究所

• Honjo Haruo

  Department Of Cardiovascular Research Research Institute Of Environmental Medicine Nagoya University

• Kamiya K

  Division Of Cardiovascular Research Reseearch Institute Of Environmental Medicine Nagoya University

• Kamiya Kaichiro

  名古屋大学環境医学研究所 循環器学

• Kamiya K

  Nagoya Univ. Nagoya Jpn

• Kamiya Kaichiro

  Department Of Humoral Regulation The Research Institute Of Environmental Medicine Nagoya University

• Uzzaman Mahmud

  Departments of Circulation and Humoral Regulation, Research Institute of Environmental Medicine, Nag

• Uzzaman Mahmud

  名古屋大学 医学系研究科循環器学

• Uzzaman M

  Departments Of Cardiovascular Research Nagoya University

• Mahmud Uzzaman

  名古屋大学環境医学研究所

• Takagishi Yoshiko

  Department of Teratology and Genetics, Research Institute of Environmental Medicine, Nagoya Universi

• Sasano Chieko

  Department of Circulation, Research Institute of Environmental Medicine, Nagoya University

• MURATA Yoshiharu

  Department of Teratology and Genetics, Research Institute of Environmental Medicine, Nagoya Universi

• Sasano Chieko

  名古屋大学 医学系研究科循環器学

• Sasano C

  Departments Of Cardiovascular Research Nagoya University

• 高岸 芳子

  名古屋大学環境医学研究所

• Takagishi Yoshiko

  Department Of Teratology And Genetics Research Institute Of Environmental Medicine Nagoya University

• Takeuchi Yoko

  Department Of Genetics Research Institute Of Environmental Medicine

• Takagishi Y

  Department Of Anatomy Mie University School Of Medicine

• Takagishi Yoshiko

  Research Institute Of Environmental Medicine Nagoya University

• Emdad Luni

  Departments of Cardiovascular Research, Nagoya University

• Shimizu Atsuya

  Departments of Cardiovascular Research, Nagoya University

• Murata Yoshiharu

  Department Of Civil Engineering College Of Engineering Nihon University

• Murata Y

  Department Of Teratology And Genetics Research Institute Of Environmental Medicine Nagoya University

• Emdad L

  Departments Of Cardiovascular Research Nagoya University

• Emdad Luni

  名古屋大学 医学系研究科循環器学

• Honjo Haruo

  Department Of Applied Physics Faculty Of Engineering

• Shimizu Atsuya

  名古屋大学 医学系研究科循環器学

• Shimizu Atsuya

  Departments Of Cardiovascular Research Nagoya University

• Takagishi Yoshiko

  Department Of Anatomy Mie University School Of Medicine

• Takagishi Yoshiko

  Department Of Teratology And Genetics Division Of Molecular And Cellular Adaptation Research Institu

• Murata Yoshiharu

  Departments Of Genetics Research Institute Of Environmental Medicine Nagoya University

• Kamiya Kaichiro

  Department Of Cardiovascular Research Research Institute Of Environmental Medicine Nagoya University

• Kamiya Kaichiro

  Division Of Circulation And Respiration Research Institute Of Environmental Medicine Nagoya Universi

• Murata Yoshiharu

  名古屋大学環境医学研究所 発生･遺伝学

• Yoshiga Masahiko

  The Department Of Cardiovascular Medicine Medical Bioregulation Yamaguchi University Graduate School

• Murata Yoshiharu

  Department Of Endocrinology And Metabolism Research Institute Of Environmental Medicine

• Yamazaki Masatoshi

  Division Of Cardiology Shizuoka Saiseikai General Hospital

• Kodama Itsuo

  Department Of Cardiovascular Research R.i.e.m. Nagoya University

• Yamazaki Masatoshi

  Research Institute Of Environmental Medicine Nagoya University

• Murata Y

  Nagoya Univ. Nagoya‐shi

• Uzzaman Mahmud

  Departments Of Cardiovascular Research Nagoya University

• Shimizu Atsuya

  Department Of Humoral Regulation Research Institute Of Environmental Medicine Nagoya University

• Emdad Luni

  Departments Of Cardiovascular Research Nagoya University

• Yamazaki Masatoshi

  Division Of Cardiology Department Of Medicine And Clinical Science Yamaguchi University Graduate Sch

• Yamamoto Masahiro

  Yokohama City Cerebrovascular Center Yokohama Japan

• 本荘 晴朗

  Department Of Cardiovascular Research Research Institute Of Environmental Medicine Nagoya University

• Takagishi Y

  Department Of Cardiovascular Research Research Institute Of Environmental Medicine Nagoya University

• Yoshida Masaaki

  Division Of Cardiology Department Of Medicine And Clinical Science Yamaguchi University Graduate School Of Medicine

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