MONOCLONAL ANTIBODIES TO HUMAN CHORIONIC GONADOTROPIN AND ITS BETA-SUBUNIT
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概要
- 論文の詳細を見る
細胞融合法により,抗hCGおよび抗β-hCGマウスモノクローナル抗体の作製を試みた.hCG又はβ-hCG免疫マウスの脾細胞と,骨髄腫株NS-Iを,ポリエチレングリコールを用いて融合させ,抗体の検出には,抗マウスIgG家兎抗体酵素複合体を利用する酵素免疫測定法(EIA)を採用した.限界希釈法によるクローニングを2回以上行ない,抗hCG系2株(4H10,4G2),抗β-hCG系1株(1D12)を得た.4H10抗体は,EIAではhCG,α-hCG,hLHと結合したが,^<125>I-hCGとは結合しなかった.また4G2抗体は,EIAではhCG,β-hCGと反応したが,^<125>I-β-hCGとは結合しなかった.抗β-hCG,1D12抗体は,β-hCG,^<125>I-β-hCGとは高い結合活性を示したが,hCG,^<125>I-hCGとは,ほとんど反応しなかった.以上の結果から,モノクローナル抗体の中には,アイソトープ標識した抗原とは結合しない場合があり,測定系に応用する際には留意する必要があると考えられた.また,抗β-hCG,1D12抗体は,hCG分子内では隠されている,β-hCGに特異的な抗原決定基を認識していると推察された.この抗体を利用したEIAでは,β-hCGに対しては,10pg/tubeの感度が得られ,hCGの交叉反応は,2.5%であった.
- 社団法人日本産科婦人科学会の論文
- 1983-12-01
著者
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HATTORI Sen-ei
Department of Obstetrics and Gynecology, Ogaki Municipal Hospital
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FURUHASHI Yoshihito
Department of Obstetrics and Gynecology, Okazaki City Hospital
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Tomoda Yutaka
Department Of Obstetrics And Gynecology Nagoya University School Of Medicine
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Tomoda Yutaka
Department Of Biopharmaceutics Kyoto Pharmaceutical University
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Hattori Senei
Department Of Obsetrics And Gynecology School Of Medicine Nagoya University
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Hattori Sen-ei
Department Of Obstetrics And Gynecology Ogaki Municipal Hospital
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Furuhashi Yoshihito
Department Of Obstetrics And Gynecology Nagoya University School Of Medicine
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GOTO Setsuko
Department of Obsetrics and Gynecology, School of Medicine, Nagoya University
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MANO Hisao
Department of Obstetrics and Gynecology Nagoya University School of Medicine
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Goto Setsuko
Department Of Obstetrics And Gynecology Nagoya University School Of Medicine
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Tomoda Y
Nagoya Univ. Nagoya Jpn
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Tomoda Yutaka
Department Of Obsetrics And Gynecology School Of Medicine Nagoya University
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