68 トマトの根が生産するヤセウツボ種子発芽誘導物質

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Tomato is a host plant of Orobanche species, the root parasite weeds. We found that roots of hydroponically-grown tomato (Lycopersicon esculentum) produce abundant Orobanche minor seed germination stimulants. Reversed phase (C_<18>) HPLC analysis of the root exudate revealed the presence of biologically active fractions, Ly1 (fast moving) and Ly2 (slow moving), the latter being a major fraction (Fig. 1). Further HPLC of Ly1 on a phenyl column separated three stimulants Ly1-a, Ly1-b and Ly1-c which seem to be novel strigolactones because their HPLC retention times are distinct from known strigolactones. The same HPLC of Ly2 gave rise to Ly2-a and Ly2-b (Fig.2,Fig3), the latter having a major biological activity. Ly2-b constitutes a wide peak with biological activity and seems to be composed of three or more compounds. The present study showed that tomato produced 6 or more strigolactones.
2002-10-31