90 エンドウ種子の成熟過程におけるブラシノステロイド生合成・代謝遺伝子の発現調節

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The amounts of brassinolide and castasterone, biologically active brassinosteroids (BRs), in pea (Pisum sativum L.) seeds increased as seeds matured and then decreased in the fully expanded seeds. The level of 6-deoxocastasterone, however, increased in the fully expanded seeds. All of these BRs were diminished in the dry mature seed. Thus, in pea seeds, the D gene ortholog seems to be involved in the increased levels of biologically active BRs, while the BAS1 gene ortholog is likely to be responsible for the decreased levels of BRs. In this study, we attempted to clone pea orthologs of the BR biosynthesis genes D, CPD and DWF4,as well as the metabolism gene BAS1. These genes were amplified by PCR using primers designed from the highly conserved sequences of legume ESTs. Cloned fragments of pea D and BAS1 were found to encode proteins that has 89% similarity with tomato D and 89% similarity with Arabidopsis BAS1,respectively. Currently, sequencing of putative CPD and DWF4 fragments is in progress. The correlation between expressions of these genes and endogenous BRs levels at several growth stages of pea seeds will be reported.
植物化学調節学会の論文
2002-10-31