15 推定プロゲステロン結合タンパク質をコードするイネ遺伝子のクローニング

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Progesterone is an animal steroid hormone that functions as a corpus luteum hormone, which maintains pregnancy and implantation of fertilized egg. In 1968,apple seeds were found to contain progesterone. Recently, we identified progesterone from Arabidopsis and pea, suggesting that progesterone is a ubiquitous constituent of plants. The GenBank database shows that various plants contain genes homologous to the animal progesterone-binding proteins (PBs). We report here the RT-PCR-based isolation of three rice genes (OsPB1,OsPB2 and OsPB 3) analogous to human progesterone-binding protein genes, their ORF sizes being 976bp, 893bp and 456bp, respectively. The amino acid sequences of these proteins indicate the presence of a putative progesterone-binding domain. OsPB1 and OsPB2 have a transmembrane domain at the N-terminal, indicative of their localization in membranes. OsPB3 has no transmembrane domain and may not be associated to membranes. The progesterone-binding domains of OsPB1,OsPB2 and OsPB3 have 66%, 66%, 59% similarities to those of human progesterone membrane binding protein, respectively. The similarities of the progesterone-binding domains between these proteins are 89% between OsPB1 and OsPB2,69% between OsPB1 and OsPB3,and 68% between OsPB2 and OsPB3. It was found that these three OsPB genes are expressed highly throughout the rice plant (leaf blade, sheath, and spike), suggesting that OsPBs, as well as progesterone, may have physiological functions.
植物化学調節学会の論文
2002-10-31

15 推定プロゲステロン結合タンパク質をコードするイネ遺伝子のクローニング

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