64 LC/MS/MS によるストリゴラクトン類(寄生雑草発芽刺激物質)の同定・定量

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Witchweeds (Striga spp.) and broomrapes (Orobanche spp.) are two most devastating root parasitic weeds causing sever damage to agricultural production. Seeds of Striga and Orobanche germinate only when they receive chemical signals, i.e., germination stimulants, produced by and released from roots of host or non-host plants. So far, four different germination stimulants (Fig. 1), strigol (1), sorgolactone (2), alectrol (3) and orobanchol (4), collectively called strigolactones, have been isolated and characterized. These strigolactones induce significant germination of Striga and Orobanche seeds at as low as 10^<-10> M and thus the amounts of strigolactones in root exudates have been estimated by bioassays. However, root exudates may contain germination inhibitors as well and thus the amounts of strigolactones might have been underestimated. Therefore, we developed a rapid and sensitive analytical method for strigolactones by high performance liquid chromatography connected to tandem mass spectrometry (LC/MS/MS). In this study, three authentic strigolactones were separated clearly on MRM (Fig. 2). Using this method, we confirmed that red clover (Trifolium pratense cv. Hamidori), a host of O. minor, produces orobanchol and alectrol but not strigol nor sorgolactone (Fig. 3). Each red clover seedling produced ca. 13,70,58,and 65 pg of orobanchol for the 1st, 2nd, 3rd and 4th weeks after germination.
植物化学調節学会の論文
2002-10-31