65.根寄生植物-宿主植物間相互作用の分子解析(III) : ストライガの寄生に対して感受性が異なるソルガムの分子応答(口頭発表)

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Sorghum genes responsive to Striga hermonthica parasitism were isolated and their expression was analyzed. Using the suppression subtractive hybridization (SSH) strategy and the quantitative RT-PCR (qRT-PCR), 30 genes, up-regulated in response to S. hermonthica parasitism, were isolated from roots of a susceptible sorghum cultivar Abu 70. Changes in expression of each of the genes were investigated in roots and leaves obtained from the S. hermonthica-parasitized sorghum cultivars Tabat and Wad Ahmed, more and less susceptible to S. hermonthica than cv. Abu 70, respectively. The changes were studied also in roots of the three sorghum cultivars treated with salicylic acid (SA) or methyl jasmonate (MeJA). S. hermonthica parasitism induced jasmonic acid (JA)-responsive genes and suppressed SA-responsive genes in roots of cvs. Abu 70 and Tabat. In contrast, in the less susceptible cultivar Wad Ahmed, S. hermonthica parasitism induced SA-responsive genes but slightly induced JA-responsive genes. These suggest that the susceptible hosts recognize S. hermonthica parasitism as abiotic stress rather than biotic stress and that the less susceptible host recognize S. hermonthica parasitism as biotic stress. Systemic expression of a few genes was observed in leaves of the sorghum cultivars parasitized with S. hermonthica.
植物化学調節学会の論文
2007-10-05