60. 根寄生植物ヤセウツボの発芽を阻害するノジリマイシンの作用点(口頭発表,植物化学調節学会第46回大会)

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Parasitic weeds, Striga spp. and Orobanche spp. cause serious damage to agriculture worldwide. A novel and effective strategy for parasitic weed control is desired for economical and humanitarian reasons. We focused on the germination process of parasitic weeds to find biological events specific to these species and conducted metabolic profiling of Orobanche minor seeds. Consequently, we revealed that gentianose, a rare trisaccharide consisted of two glucose and a fructose, was decreased concurrently with increases of glucose and fructose immediately after GR24 treatment. A potent inhibitor of glycosyl hydrolases, nojirimycin bisulfite, decreased not only the germination rate of O. minor but also that of Striga gesnerioides. From these results, we hypothesize that gentianose metabolism is crucial for germination of these parasitic weeds and the key enzyme in the gentianose metabolic pathway could be a novel target for selective control of parasitic weeds. To characterize the enzymes involved in gentianose metabolism, we extracted crude enzyme from O. minor seeds and measured glycosyl hydrolyzing activity. As a result, it is revealed gentianose is hydrolyzed to monosaccharides via disaccharide gentiobiose. The gentiobiose hydrolyzing activity in the cell wall/membrane binding protein fraction was increased during the germination process and reached a maximum 4 days after GR24 treatment. Additionally, gentiobiose hydrolyzing enzyme was inhibited selectively by nojirimycin.
植物化学調節学会の論文
2011-10-03

60. 根寄生植物ヤセウツボの発芽を阻害するノジリマイシンの作用点(口頭発表,植物化学調節学会第46回大会)

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