32 シロイヌナズナ矮性突然変異体chibi2におけるブラシノステロイドの代謝

概要

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The Arabidopsis dwarf mutant chibi2 obtained by enhance tagging is brassinosteroid (BR)-deficient due to overexpressed cytochrome P450 (CYP72C1) that deactivates endogenous BRs. In this study metabolism of tritiated castasterone (CS) and brassinolide (BL) fed to suspension cultures of chibi2 and WT seedlings was examined. Metabolites obtained from seedlings incubated for 1,2 of 3 days were fractionated into acidic and neutral fractions prior to radioactivity measurement. No clear difference between chibi2 and WT was observed in all incubations, indicating that metabolism of upstream BRs prior to CS may be enhanced in chibi2. However, HPLC analysis followed by GC-MS revealed the presence of BL in the 3-day incubation of chibi2 but not wild type, indicating that lactonization is also activated in prolonged incubation of chibi2 with CS.
植物化学調節学会の論文
2000-11-02