18 エンドウの根が分泌するヤセウツボ種子の発芽誘導物質

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A variety of leguminous plants are parasitized by Orobanche species, the root parasitic weeds. We found that roots of hydroponically-grown garden pea (Pisum sativum) produced abundant Orobanche minor seed germination stimulants. Reversed phase (C_<18>) HPLC analysis of the root exude revealed the presence of two major germination stimulants 1 (fast moving) and 2 (slow moving). Further HPLC on reversed phase (Phenyl) and silica gel (Aquasil) yielded UV-spectrometrically pure 1 and 2 whose UV λmax was ca 280 nm, indicative of these being strigolactones. These stimulants seem to have novel structures because their HPLC retention times are distinct from known strigolactones. The yields of compounds 1 and 2 from 276-liter culture medium were ca 0.2 and 100μg strigol equivalents, respectively.
植物化学調節学会の論文
2000-11-02