10 エンドウLKaの遺伝子はブラシノステロイド受容体をコードしている

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The dwarf mutant lka of garden pea (Pisum sativum L.) has reduced sensitivity to brassinosteroids and increased levels of endogenous brassinosteroids, suggesting that Lka dwarfs are defective in the perception or signal transduction pathway of brassinosteroids. We show that the Lka gene is a pea ortholog of the Arabidopsis BRII gene encoding a leucine-rich repeat kinase which functions as a brassinosteroid receptor. Low-stringency screening of a pea cDNA library with the Bill cDNA probe produced a full-length BRIIhomolog (termed PsBRII). The predicted amino acid sequence of PsBRI1 shows 78% similarity (68% identity) with that of the BRII gene. Sequence analysis indicated that the PsBRII gene in lka plants has a single base substitution resulting in the missense of highly conserved aspartic acid into asparagine just before brassinosteroid-binding region. Furthermore, co-segregation between the RFLP and the tall/dwarf phenotype strongly implied that the mutation results in the dwarf phenotype of the mutant lka plant. Thus, the PsBRII gene functions as a brassinosteroid receptor kinase in pea and can be mapped to the Lka locus. We also report the northern analyses of the PsBRII gene.
植物化学調節学会の論文
2001-10-09