テオフィリンの体液中濃度測定におけるHomogeneous Enzyme Immunoassayを応用したSyva System (AC-5000)の評価

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The applicability of recently developed instrument, automatic Syva System AC-5000 (EMIT-AC), was evaluated. The concentration of total or free theophylline (TH) in plasma, serum, saliva, and spiked sample was measured by EMIT-AC. The results were compared with those obtained by high performance liquid chromatography (HPLC) or substrate-labeled fluorescent immunoassay (SLFIA). There was no significant difference (P>0.05) between the results obtained by EMIT-AC and HPLC method using TH spiked samples. The within-day precision of EMIT-AC method was less than 4.5% as the coefficient of variation (C.V). The C.V. value for between-day precision was 6.3% for EMIT-AC and 3.7% for HPLC method and no significant difference (P>0.05) was observed between the mean values. A good correlation was-obtained between EMIT-AC and HPLC method (n=54, r=0.988, P<0.001 for plasma n=22, r=0.998, P<0.001 for serum ) in the assay of total TH level. Similar observation was obtained in the free level assay. It was clarified that saliva level can also be analyzed by EMIT-AC method as well as SLFIA method. These findings show that EMIT-AC method is a satisfactory analytical device for giving correct results and applicable for the determination of TH level in body fluid especially in clinical drug monitoring requiring rapidity.
1984-06-20