9-Theophylline-BSA抗血清を用いたSubstrate-Labeled Fluorescent Immunoassayの臨床への適用

概要

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Theophylline concentration in plasma sampled from asthmatic patients was determined by newly developed substrate-labeled fluorescent immunoassay (9-SLFIA) method using antiserum produced from 9-theophylline-BSA immunogen with low cross-reactivity to caffeine. The results of this method were compared with those obtained by high-performance liquid chromatography and substrate-labeled fluorescent immunoassay (8-SLFIA). The correlation coefficients of the results obtained by 9-SLFIA and the high-performance liquid chromatography (r=0.985), 8-SLFIA(r=0.977) showed good agreement. Cross-reactivity, as determined by the amount of compound required to increase the measured theophylline concentration by 20% when added to a 15 μg/ml theophylline sample, was 100μg/ml for caffeine, 10μg/ml for 8-chlorotheophylline, 1000 μg/ml for theobromine and 1000 μg/ml for 3-methylxanthine. The variation coefficient, of the replicate analysis by 9-SLFIA on normal human plasma samples with known theophylline concentrations were below 10%.
1983-06-20