SLFIA法およびEMIT法により測定した血中テオフィリン濃度の比較 : 小児科領域への応用

概要

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Theophylline concentration in plasma collected from asthmatic patients was determined by Substrate-Labeled Fluorescent Immunoassay (SLFIA, AMES TDA). The results of this method were compared with those of Homogeneous Enzyme Immunoassay (EMIT). High correlation was found between SLFIA and EMIT, showing the correlation coefficient 0.970 (n=47). The variation coefficient of replicate analysis on a spiked sample by the SLFIA method was below 5%. The modified SLFIA method was applied to the theophylline plasma level of a small sample for immature infants. The values of the modified SLEIA correlated well to those determined by SLFIA and EMIT. The correlation coefficients were 0.989 (n=15) between modified SLFIA and SLFIA, and was 0.964 (n=15) between the modified SLFIA and EMIT, respectively. The variation coefficient of the replicate analysis on a spiked sample by the modified SLFIA was below 8%. In view of application to pediatric patients, the procedure of the modified SLFIA has considerable advantages over hitherto available method which requires only 10μl of plasma for a single determination thanks to the elimination of the deproteinization and extraction steps.
1982-06-20