hCTと甲状腺cyclic AMP

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It is well known that a thyroid stimulator, human chorionic thyrotropin (hCT), as well as human chorionic gonadotropin is secreted from normal placental tissue. After extraction of thyrotropic and gonadotropic activities from placental powder by percolation with solutions of decreasing ethanol content, the concentrate was chromatographed on carboxymethyl cellulose (CM-cellulose) with subsequent gelfiltration of the active fractions on Sephadex G-100 and chromatography on DEAE-cellulose and DEAE-Sephadex A-50. Thyrotropic and gonadotropic activities were isolated by the procedures used in our laboratory. The steps in chromatography on CM-cellulose and DEAE-Sephadex A-50 used are important for the isolation of hCG from hCT. The potencies of the final products were 250 mU/mg of hCT and 8,200 IU/mg of hCG. Commercial hCG of urinary origin (12,000 IU/mg) was purified by a similar method of chromatography on CM-cellulose. The bulk of hCG was localized in the unadsorbed fraction (14,000 IU/mg) with conductivity of 0.5 m mho, pH 6.0 NH4Ac, and thyrotropic activity in the adsorbed fraction was eluted between conductivities of 5.2 -11.3 m mho. These results indicated that the chromatographic properties were different between hCT and hCG. On the other hand, the authors examined the effect of varying concentration of hCT and hCG on cyclic AMP levels in a guinea pig thyroid slice. An increase in the level of cyclic AMP has been observed in response to hCT, but hCG had no effect on the level of cyclic AMP in guinea pig thyroid slices. Recently, the question had been raised as to whether the thyroid stimulator found in the serum and tumor of patients with trophoblastic disease and hyperthyroidism might be hCG. The present results, however, strongly suggest that hCG is not thought to be the thyroid stimulator which has been found in normal placenta.
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hCTと甲状腺cyclic AMP

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