ヒト妊娠初期脱落膜および絨毛におけるドーパミンの測定

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Several reports revealed that catecholamine, which may play a significant part in supporting fetal growth and the function of the placenta or maintaining the pregnancy, exists in the feto-placental unit. In this experiment, we measured dopamine, one of the catecholamines, in decidual and villous tissues in early normal human pregnancies in order to elucidate the role of dopamine during pregnancy. It is difficult to measure catecholamine concentrations in tissues with high sensitivity and specificity; therefore, we utilized two methods : the fluorimetric method and high pressure liquid chromatography. The results were the following : 1) We extracted and measured dopamine from decidual and villous tissues by the fluorimetric method, and the dilution curve of the sample material was parallel with synthetic dopamine. 2) In the sixth or seventh week of pregnancy, dopamine concentrations were 110.57 ± 8.52 ng/lg wet weight (w.w.) in the decidua and 88.96 ± 20.38 ng/lg w.w. in the villi. In the former, it decreased gradually thereafter. In the latter, after it transiently increased to 130.78 ± 20.93 ng/lg w.w. in the eleventh week of pregnancy, it also decreased gradually. However, there was no significant difference between the value in the decidua and in the villi during the first trimester. 3) We extracted catecholamines from both tissues by means of high pressure liquid chromatography, and we measured dopamine and noradrenaline contents automatically by means of a fluorimetric spectromonitor. By this method, high. specific dopamine was proved to be in both tissues. The results revealed that dopamine and noradrenaline were contained in decidual as well as in villous tissues of early pregnancy.
一般社団法人日本内分泌学会の論文

ヒト妊娠初期脱落膜および絨毛におけるドーパミンの測定

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