絨毛中のImmunoreactive Luteinizing Hormone-Releasing Hormone (IR-LHRH) に関する研究

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We extracted the immunoreactive luteinizing hormone-releasing hormone (IR-LHRH) from villous and plasma in the first trimester of pregnancy with 0.2N acetic acid and the florisil absorbtion method. Recovery rate was 40.0 ± 4.08% in the former and 62.5 ± 9.5% in the latter, respectively. These IR-LHRH were measured by radioimmunoassay, which showed sensitivity from 5 to 500 pg/ml. The villous IR-LHRH was compared with synthetic LH-RH by a dilution curve in different concentrations and the column gel filtration of sephadex-G25. The dilution curve of synthetic LH-RH and sample material showed a significant correlation, and the elution time of villous IR-LHRH on the column was something faster than that of synthetic LH-RH. The concentration of villous IR-LHRH showed 20-100 pg/0.1g dry weight in the first trimester of pregnancy, and the peak was at 9 weeks of normal pregnancy and at 8 weeks of inevitable abortion respectively, but these IR-LHRH levels were not changed between pre- and post-dilation and curettage. From this evidence, we considered that IR-LHRH show activity of the local hormone in villous tissue similar to somatostatin.
日本内分泌学会の論文