コイ,ウナギおよびブリのbilirubin UDP glucuronyl transferaseの細胞内分布と酸素化学的性質〔英文〕

概要

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Intracellular distribution and enzymatic properties of bilirubin UDP glucuronyl trasferase (EC 2.4.1.76) of carp Cyprinus carpio, eel Anguilla japonica, and yellowtail Seriola quinqueradiata, were studied. The enzyme activity was highest in the microsomal fraction. The pH optima of this enzyme were 8.0, 8.0 and 8.2 for carp, eel and yellowtail, respectively. Triton X-100 effectively activated the enzyme activity in these three fishes, and the optimal concentrations were 0.2, 0.4 and 0.6% for eel, carp and yellowtail enzyme, respectively. Under the conditions used for the enzyme assay, the enzyme of yellowtail showed highest activity near 45°C and those of other two fishes showed highest activity near 37°C. Yellowtail enzyme maintained most activity at 60°C, whereas the enzymes of other two fishes were rapidly inactivated at this temperature.