OMV感染RTG-2細胞およびサケO.keta肝細胞の超微構造

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The ultrastructures of RTG-2 cells and hepatocytes of chum salmon (Oncorhynchus keta) infected with Oncorhynchus mason virus (OMV) were observed by thin sectioning and negative staining. RTG-2 cells were fixed 3 and 7 days after OMV infection (m.o.i.=0.01) and the tissue samples from the liver of moribund 80-day-old, 3-month-old and 4-month-old chum salmon infected with OMV by the immersion method (100 TCID50/ml, 60 min) were prepared for electron microscopy. The infected RTG-2 cells and hepatocytes were prefixed with paraformaldehyde-glutalaldehyde and post fixed with 2% osmic acid.Electron micrographs of both RTG-2 cells and hepatocytes infected with OMV revealed the following activities: nuclei showed the margination and the subsequent aggregation of chromatine; syncytia was found in both RTG-2 cells and hepatocytes; the nuclear membranes became smooth and the virus matured in the nucleus of those cells; the matured virus particles were observed in the vesicle and around the cell membrane; and enveloped virus particles were seen in the focus of the hepatocyte. The size of enveloped virus particles was approximately 200×240nm and the nucleocapsid was 115nm in diameter.
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