フリ-ジア"ロイヤルクラウン"の種子発芽の温度依存性

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Seed germination of freesia, Freesia hybrida cv. Royal Crown as affected by high temper-atures was investigated in reference to hastening of germination and changes in protein metabolism. 1. Optimum temperature for seed germination ranged from 15° to 20°C. Seed germina-tion was inhibited completely at 30°C, even though seeds were incubated at 20°C until just before germinated. When seeds kept at 30°C were cooled to 20°C, the inhibition of germi-nation was reversed. However, the number of days required for seeds to germinate at 20°C increased with the length of 30°C incubation period. 2. Once the seeds germinated, the growth of radicle was not inhibited at 30°C, and the maximum rate of elongation occurred at 25°C. 3. The inhibition of seed germination at 30°C was not reversed by treating seeds with the growth regulators GA3, BA, and zeatin or by soaking them in concentrated sulfuric acid. Seeds treated with sulfuric acid for 10 min germinated more rapidly and uniformly at 20°C than did not-treated seeds. 4. When embryos excised from ungerminated seeds were cultured aseptically, radicle growth occurred sooner even at 30°C; root growth was better at 30°C than at 20°C. 5. When seeds were incubated for 2 days at 20° or 30°C after 4 days incubation at 20°C, the SDS polyacrylamide gel electrophoretic patterns of embryonic proteins differed between treatment, whereas the patterns of endospermic proteins did not. When seeds were treated with [14C] -leucine at 30°C, total incorporation and incorporation into TCA-insoluble materi-als by the embryo and endosperm were less than that incorporated by seed parts kept at 20°C. The inhibition of labelled leucine uptake by the endosperm after 24 hr at 30°C was significantly large. These results suggest that elevated temperatures decelerate protein turnover which is some-how involved in the inhibition of germination of Freesia seeds.
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フリ-ジア"ロイヤルクラウン"の種子発芽の温度依存性

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