Kupffer cell-derived growth factor for fibroblasts in acute liver injury induced by carbon tetrachloride.

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To evaluate the role of Kupffer cells (KCs) in hepatic fibrosis, KCs isolated from rat liver by a collagenase perfusion method were cultured with and without a stimulant, lipopolysaccharide (LPS). Supernate from the culture of LPS-stimulated KCs contained a fibroblast growth factor (FGF) that caused a significant increase of 3T3 fibroblast DNA synthesis. Furthermore we estimated the possible role of KCs in transient hepatic fibrogenesis induced by acute carbon tetrachloride (CCl4) treated rats stimulated fibroblast proliferation to a greater degree than that of control rats. Enhanced FGF production of KCs occured within 24hr and increased 4-fold at 48hr after injury.These findings suggest that KCs modulate fibroblast growth by releasing a growth factor and play an important role in the progression of hepatic fibrosis.
財団法人日本消化器病学会の論文