The defensive effect of gastric mucus against carcinogenesis. Quantitative measurement of incorporation of N-(methyl-3H)-N'-nitro-N-nitrosoguantidine into gastric mucosal DNA.:Quantitative Measurement of Incorporation of N-[methyl-3H]-N'-nitro-N-nitrosogu

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The purpose of this study is to investigate some factors that influence the process of gastric carcinogenesis. We used rats, which N-[methyl-3H]-N'-nitro-N-nitrosoguanidine ([methyl-3H] MNNG) was given orally. The amount of imcorporated [methyl-3H] MNNG with gastric mucosal DNA was measured by a liquid scintillation counter. In control, the amount of imcorporated [methyl-3H] MNNG was 25.4± 5.9pmole/mgDNA. When prostaglandine E2 or pirenzepine, which increased gastric mucus, was administered orally prior to [methyl-3H] MNNG, the imcorporated amount was 11.7±3.8pmole/mgDNA and 6.2±5.6pmole/mgDNA respectively. On the contrary, in the administration of indomethacin, which decreased gastric mucus, the increase of incorporation up to 42.9±14.4pmole/mgDNA was measured. From these results, the negative correlation was found between the amount of gastric mucus and incorporated carcinogen. Accordingly, gastric mucus has a protective effect not only against ulcergenic agents but also carcinogens. So it is suggested that gastric mucus plays an important role in the defensive mechanism against carcinogenesis.
財団法人日本消化器病学会の論文