スクシンイミドエステルの化学 (第10報) : N-スクシンイミジルベンゾエートでラベル化した脂肪族第一級アミンの逆相HPLCによる分析

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The reactions of N-succinimidyl benzoates (1) with aliphatic primary amines (2) in acetonitrile or dioxane gave the corresponding N- (arylcarbonyl) amines (3). Reverse phase HPLC analysis of (3) was performed at 210300nm detection wavelengths using acetonitrile containing 070vol% of water as the mobile phase. Plots of log (corrected retention volume : V) vs. carbon number (CN) of alkyl group in (3) showed good linear relationship. The slope of the log V vs. CN plot increased with water content in the mobile phase. An interaction between the aryl group in (3) and the reverse phase appeared to occur in consideration of the result in which it was affected by water. The relative response of (3) at 254nm was found to be correlated linearly with the molar absorptivity of (3) at 254nm. The separation of labelled butylamines was also examined.
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