スクシンイミドエステルの化学 (第15報) *1 : N-スクシンイミジルベンゾエートの逆相HPLC分析用ラベル化剤としての応用脂肪族アミンの分離と合成ジヒドロスフィンゴシンのエリトロ体含量の定量

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Reverse phase HPLC analysis of amino compounds labeled with N-succinimidyl benzoates (1) was carried out. Aliphatic primary and secondary amines labeled with N-succinimidyl pnitrobenzoate (1) (X=p-NO2) could be separated by using aqueous acetonitrile containing 0.1% phosphoric acid as an eluent. The secondary amines with 1-branched chains could not be detected since they did not react with (1) (X=p-NO2). Good erythro/threo separation of synthetic dihydrosphingosines (4) labeled with (1) was observed, and the erythro content was about 70%, which is in good agreement with that determined by the previously reported GLC method.