蛍光検出高速液体クロマトグラフィーによるヒト胎盤中のビタミンK類の測定

概要

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Endogenous K vitamins (VKs) in human placentae were detected and identified by high-performance liquid chromatography (HPLC) with fluorometric detection using post-column reduction. Phylloquinone (K1) and menaquinones (K2: MK-4, 6, 7) were found in human placentae.The recovery rates of this assay were assessed by adding known amounts of VKs to the sample. Between 60% and 65% of added K1 and MK-4, 6, 7 were recoverd from the placentae by this assay method.The mean placenta concentration of K1 in 13 normal placenta with normal diet was 1.22ng/g (range: 0.55-2.29), MK-4 was 1.14ng/g (range: 0.38-2.04), MK-6 was 0.13ng/g (range: not detected-0.61), MK-7 was 1.04ng/g (range: 0.36-2.55). These data were corrected by recovery rates. The detection limits of K1 and MK-4 in placentae were 0.1ng/g, and those of MK-6 and MK-7 were 0.2ng/g.Two pregnant women ate fermented soybeans (NATTO) on alternate days for one month before delivery. Their placentae contained a high density of MK-7 (6.69 and 14.95ng/g), because MK-7 is abundant in fermented soybeans.These results may be helpful to elucidate the cause of a neonatal hemorrhagic disease occuring two or three days after birth which appears to be at least in part responsive to vitamin K therapy.
日本衛生学会の論文