ウレアーゼによる尿素の加水分解を利用する均一溶液からのマンガン(II)-オキシネートの沈殿

概要

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Hydrolysis of urea with urease was applied to the precipitation of Mn (II)-oxinate from homogeneous solution and the optimum conditions for the quantitative precipitation of the oxinate were determined. To the solution containing 650mg of manganese (II) were added 100mg of ascorbic acid, 1ml of 10% citric acid, 50ml of 20% urea solution and an alcoholic solution of oxine in 20% excess. The pH of the solution was adjusted to 5.0 with ammonia water and then 0.3mg of urease was added to the solution. The mixture was kept at 35°C for 610hours in a thermostatic water bath. The precipitate formed was filtered, washed, dried at 170°C for one hour, and weighed as Mn (C9H6ON)2. It was found that urease was co-precipitated almost completely with manganese oxinate. Manganese was determined with satisfactory result by deducting the weight of urease used from the weight of the precipitate.
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