Light and electron microscopic observation of lymphocytes subsets in liver of patients with primary biliary cirrhosis by immuno-peroxidase method using monoclonal antibodies.

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The distribution of lymphocytes subsets was studied by the peroxidase-labeled antibody method using mouse monoclonal antibodies against Leu-1, Leu-2a, Leu-3a, Leu-7 and Leu 10 antigens in liver biopsies from 5 patients with primary biliary cirrhosis (PBC) at the light and electron microscopic levels. Leu-1+ cells (pan T cells), especially Leu-2a+ cells (cytotoxic/suppressor T cells), were frequently demonstrated in interlobular bile ducts with chronic nonsupprative destructive cholangitis (CNSDC). In the ultrastructural observation, Leu-1+ cells and Leu-2a+ cells often migrated into the space between epithelial cells of bile ducts. These lymphocytes often showed broad surface contact against bile duct epithelial cells. Some epithelial cells in contact with lymphocytes showed loss of microvilli or bleb formation on the laminal surface. These findings suggest that cytotoxic T lymphocytes may be associated with CNSDC in PBC.
社団法人日本肝臓学会の論文