Collagen fiber production by Ito cells in human hepatic fibrosis. A light and electron microscopic autoradiographic study.:A light and electron microscopic autoradiographic study

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To investigate the collagen-producing capacity of Ito cells in human hepatic fibrosis, biopsized liver tissues were examined by light and electron microscopic autoradiography using 3H-proline as a tracer. Following biopsies, liver specimens (22cases) were trimmed immediately and incubated at 37°C for 1 hour in the medium containing 200μCi/ml 3H-proline and then chased for 6 hours. After fixation and epon-embedding, thin sections were prepared for light and electron microscopic autoradiography (2-6 Weeks exposure). After 1 hours labelling, numerous silver grains were seen over fibldblasts and Ito cells particularly located in piecemeal necrosis, in addition to over hepatocytes and bile ducts. After a 6 hour chase, silver grains were also over collagen fibrils adjacent to the labelled Ito cells and fibroblasts, indicating incorporation of proline into these cells, and subsequent synthesis and Secretion of collagen. From these results, Ito cells can produce collagen fibers in human hepatic fibrosis, as do fibroblasts in the portal tract.