Leakage of the fluorescent Ca2+ indicator fura-2 in smooth muscle.
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概要
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The movement of a fluorescent intracellular Ca<SUP>2+</SUP> indicator, fura-2, in smooth muscle was examined. Strips of rat and rabbit aortas and bovine trachea were loaded with the acetoxymethyl ester of fura2 (fura-2/AM), followed by washing with normal physiological solution. Not only fura-2/AM but also fura-2 was detected in the washout solution. The amount of fura-2 in the cells, measured fluorometrically, decreased gradually during the washout. The decrease was fastest in rat aorta followed by rabbit aorta > bovine trachea. In rat aorta, fura-2 leakage was inhibited by an inhibitor of anion transport, probenecid, or by a decrease in bath temperature. The Ca<SUP>2+</SUP> ionophore ionomycin (10 μM)increased the leakage of fura-2, which was not inhibited by probenecid, possibly because a high concentration of ionomycin nonselectively increased membrane permeability. These results suggest that fura-2/AM is cleaved to fura-2 in the cell which gradually leaked out of the cell mainly by an anion transport system. The amount of fura-2 in the cell seemed to be determined mainly by the rate of leakage of fura-2, which is the largest in rat aorta followed by rabbit aorta and bovine trachea.
- 公益社団法人 日本薬理学会の論文
著者
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Abe Asaki
Department Of Animal Science And Technology Faculty Of Agriculture Okayama University
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Mitsui Minori
Department Of Veterinary Pharmacology Faculty Of Agriculture The University Of Tokyo
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Karaki Hideaki
Department of Veterinary Medical Sciences, Faculty of Agriculture, The University of Tokyo
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Tajimi Masaomi
Department of Veterinary Pharmacology, Faculty of Agriculture, The University of Tokyo
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