Inhibitory effects of forskolin on vascular smooth muscle of rabbit aorta.
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概要
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Effects of forskolin on the contractions in rabbit aorta were examined. The sustained contraction induced by 10<SUP>-6</SUP> M norepinephrine was inhibited by 10<SUP>-8</SUP>-10<SUP>-5</SUP> M forskolin in a concentration-dependent manner. In the high K<SUP>+</SUP>-depolarized and verapamil-treated aorta, the norepinephrine-induced sustained contraction was similarly inhibited by forskolin. However, forskolin showed only a slight inhibitory effect on the sustained contraction induced by 65.4 mM KCl. Forskolin inhibited the increase in Ca<SUP>2+</SUP> influx due to norepinephriiie, but not that due to high K<SUP>+</SUP>. In a Ca<SUP>2+</SUP>-free solution, 10<SUP>-6</SUP> M norepinephrine induced a transient contraction which is due to Ca<SUP>2+</SUP> release from the store site. This contraction was inhibited by 3×10<SUP>-7</SUP>-10<SUP>-5</SUP> M forskolin. However, caffeine-induced transient contraction was not inhibited by 10<SUP>-5</SUP> M forskolin. <SUP>45</SUP>Ca<SUP>2+</SUP> in a cellular site was released by 10<SUP>-6</SUP> M norepinephrine or 10 mM caffeine. Forskolin inhibited the Ca<SUP>2+</SUP> release induced by norepinephrine, but not that by caffeine. Forskolin increased the tissue cAMP content in resting, 10<SUP>-6</SUP> M norepinephrine-treated or 65.4 mM K<SUP>+</SUP>-treated aorta. It is concluded that forskolin inhibits the norepinephrine-induced sustained contraction by relatively selectively inhibiting the receptor-linked Ca<SUP>2+</SUP> channel, and it inhibits the norepinephrine-induced transient contraction by inhibiting Ca<SUP>2+</SUP> release from the cellular store.
- 公益社団法人 日本薬理学会の論文
著者
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Abe Asaki
Department Of Animal Science And Technology Faculty Of Agriculture Okayama University
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Karaki Hideaki
Department of Veterinary Medical Sciences, Faculty of Agriculture, The University of Tokyo
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